Turn on all 3 gasses by turning the valve at the top of the tanks. He, then Air and H2 last.
Turn on the GC using the white button at the bottom of the instrument.
On the computer, click on GC Icon on the windows screen.
Wait until the unit indicates that it is ready (Flame has to light, the injector and the oven have to warm up).
Under Methods, Select the method you are going to use or edit the parameters for a new method.
Typical parameters follow, enter them as the experiment suggests:
Column: 5% Phenyl Methyl Polysiloxane
Dimensions: 30m x 0.32mm x 0.25microns thick coating
Carrier Gas: Helium
Carrier Gas Flow Rate: 2 mL/min
Injector Temp: 280° C
Column Temperature program: 30° C hold for 2 minutes
Then ramp to 150° C, at a rate of 10° C/min
Detector Gasses: H2 and Air flame, with Helium make-up gas
Injection amount: 1ml
Total time for the run: about 30 minutes.
Place your vials in the autosampler, noting their position
Go to the Sequence Table
Enter the position (Vial #) and name of your samples, the method etc as shown on the table.
Save the sequence with a unique name.
Run the sequence.